Comparing the Efficacy of Different Anticoagulants in Blood Sample Stability for Hematological Analysis: A Quantitative Assessment

Authors: Abdulaziz M. Alangari, Abdullah M. Alenazi, Dima A. Alharbi, Sarah A. Alanazi

DOI: https://doi.org/10.5281/zenodo.13846189

Short DOI: https://doi.org/g5vwkg

Country: Saudi Arabia

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Abstract: Background: Accurate hematological analysis relies on the stability of blood samples, which is affected by the type of anticoagulant used. This study compares the efficacy of EDTA, sodium citrate, and heparin in preserving blood sample stability over time for key hematological parameters.

Methods: A total of 60 healthy volunteers provided blood samples, which were treated with EDTA, sodium citrate, or heparin. Hematological parameters, including platelet count, white blood cell count, and hemoglobin levels, were measured at four intervals: baseline, 6 hours, 24 hours, and 48 hours post-collection.

Results: EDTA and sodium citrate preserved sample stability effectively up to 48 hours, particularly for platelet and white blood cell counts. Heparin showed significant degradation in platelet and white blood cell stability by 24 hours, with substantial platelet clumping observed at 48 hours.

Conclusions: EDTA and sodium citrate are superior anticoagulants for preserving blood sample stability for hematological testing, while heparin is less effective, particularly for tests involving platelet and white blood cell counts.

Keywords: Hematological analysis, anticoagulants, blood sample stability, EDTA, sodium citrate, heparin

Paper Id: 231252

Published On: 2014-06-04

Published In: Volume 2, Issue 3, May-June 2014

Cite This: Comparing the Efficacy of Different Anticoagulants in Blood Sample Stability for Hematological Analysis: A Quantitative Assessment - Abdulaziz M. Alangari, Abdullah M. Alenazi, Dima A. Alharbi, Sarah A. Alanazi - IJIRMPS Volume 2, Issue 3, May-June 2014. DOI 10.5281/zenodo.13846189

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