IN VITRO STUDIES OF ANTI BACTERIAL ACTIVITY BRAHMI AGAINST E. COLI

Authors: Mahesh babu Guttula, Sai Priya Chennamsetti, Sai Prasad Ranastula, Juhi Karishima Mohammad, Jyothirmai Rakurthi, Sumana Sri Nadimpalli, Mary Grace Pandu, Praveen Gujjula

Country: India

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Abstract: Brahmi (Bacopa monnieri), a well-known medicinal herb in Ayurveda, is widely used for its neuroprotective, antioxidant, and anti-inflammatory properties. Recent studies suggest that Brahmi also exhibits promising antimicrobial activity against various bacterial pathogens. The present study aimed to evaluate the in vitro antibacterial potential of Brahmi extracts against Escherichia coli (E. coli), a gram-negative bacterium responsible for various gastrointestinal and urinary tract infections. Ethanolic extract demonstrated higher antibacterial activity than the aqueous extract, likely due to the higher solubility of active phytochemicals such as alkaloids, saponins, and flavonoids in organic solvents. The zone of inhibition increased with an increase in extract concentration, indicating a dose-dependent response. MIC and MBC values showed that ethanolic extract exhibited greater efficacy in inhibiting and killing E. coli. Compared to the standard antibiotic (Ciprofloxacin), the Brahmi extract showed moderate antibacterial activity, making it a potential alternative for managing bacterial infections. Brahmi extracts, particularly the ethanolic extract, exhibit significant antibacterial activity against E. coli. The study demonstrated that Brahmi could be a promising natural antibacterial agent. Further studies are recommended to isolate and characterize the active constituents and evaluate their pharmacological potential. Brahmi (Bacopa monnieri) showed significant antibacterial activity against E. coli, with the ethanolic extract being more effective than the aqueous extract. The study highlights the potential of Brahmi as a natural antimicrobial agent that may help combat antibiotic resistance.

Keywords: Invitro Studies, Anti-Bacterial Activity, Brahmi, E.coli

Paper Id: 232307

Published On: 2025-03-23

Published In: Volume 13, Issue 2, March-April 2025

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